Resumo Em razão dos graves efeitos colaterais causados pela maioria dos medicamentos antidiabéticos atualmente utilizados, continua a busca por novos medicamentos seguros para o controle do diabetes. As naftoquinonas, que possuem fortes propriedades antioxidantes, têm sido empregadas como candidatas à terapia do diabetes. O presente estudo visa encontrar o potencial antioxidante e hipoglicemiante de alguns novos derivados de 2-fenilamino-1,4-naftoquinonas (PAN), incluindo derivados de cloro, nitro, metil e bromo (5a-d) sintetizados por experimento em pote único. Os cristais do produto foram purificados por TLC e caracterizados por FT-IR. O potencial antioxidante dos compostos foi testado por meio de atividades de sequestro de radicais DPPH e redução de energia observada como absorção no UV-vis. O ensaio DPPH mostrou a poderosa atividade antioxidante dos derivados nitro e bromo, enquanto o derivado nitro mostrou o potencial de redução significativo para o ensaio FRAP. O potencial hipoglicêmico dos compostos foi estudado em modelo animal de rato. Todos os compostos sintetizados revelaram melhor atividade hipoglicemiante; no entanto, o derivado cloro apresentou atividade hipoglicêmica mais potente, com redução de 43% nos níveis médios de glicose no sangue dos animais tratados. Como a biorredução de naftoquinonas pode ser influenciada pela alteração de suas propriedades redox, notou-se que o efeito da doação eletrônica por ressonância (+R) do grupo “cloro” tem sido significativo na atividade biológica por meio da estabilização de sua forma imina, que limita o potencial de geração de radicais livres durante a biorredução de quinonas, e, portanto, tem sido proposto como a razão de sua atividade hipoglicemiante. Estudos futuros empregando as propriedades de grupos de doação eletrônica de PAN podem otimizar a interação droga-receptor para melhor planejamento de medicamentos e estratégias de desenvolvimento de medicamentos contra o diabetes e também para os ensaios clínicos.

Abstract Due to the severe side effects revealed by most of the currently used antidiabetic medicines, search for finding new and safe drugs to manage diabetes is continued. Naphthoquinones possessing strong antioxidant properties have been employed as candidates for diabetes therapy. Present study is aimed at finding the antioxidant and hypoglycaemic potential of some novel derivatives of 2-phenylamino-1,4-naphthoquinones (PAN) including chloro, nitro, methyl and bromo (5a-d) derivatives synthesized by single pot experiment. Product crystals were purified by TLC and characterized by FT-IR. The antioxidant potential of the compounds was assayed through DPPH radical scavenging and reducing power activities noted as UV-vis. absorbance. The DPPH assay has showed the powerful antioxidant activity of nitro and bromo derivatives, while the nitro derivative showed the significant reduction potential towards FRAP assay. Hypoglycaemic potential of the compounds was studied in rat animal model. All synthesized compounds revealed better hypoglycaemic activity; however, the chloro-derivative exhibited the more potent hypoglycaemic activity showing about 43% reduction in the mean blood glucose levels of the treated animals. As the bioreduction of naphthoquinones may be influenced by changing its redox properties, it has been noticed that the e-donating resonance effect (+R) of ‘chloro’ group has shown the significant effects on biological activity through stabalization of its imine form which limits the potential of generation of free radicals during bioreduction of quinones and thus has been proposed as the reason of its hypoglycaemic activity. Future studies employing the properties of e-donating groups of PAN may optimize the drug-receptor interaction for better drug designing and drug development strategies against diabetes and also for the clinical trials.

Salinity-fertility interaction has not been properly explored especially in saline-sodic soils. Therefore, the current study investigated the response of wheat (Triticum aestivum L.) to saline irrigation water in saline-sodic soil supplemented with potassium (K) and phosphorus (P). Wheat was grown in pots filled with saline-sodic soil which were irrigated either with normal or saline water. Potassium and P fertilizers were applied at two levels. Results indicated that growth of wheat plants was impaired by saline irrigation resulting in a decreased grain and dry matter yield. The P application had significant effect on the dry matter yield and other yield components. Potassium application significantly increased dry matter yield and root mass. Wheat yield increased by 14 and 7% with the application of 150 kg K2O ha-1 and 120 kg P2O5 ha-1 over the control soil under saline irrigation. The higher yield was obtained with combined P and K treatment under non-saline irrigation. The P addition significantly affected shoot and root [P] and [Na], [K] and [Mg] in the shoot tissue while the K:Na ratio produced non-significant effects under both irrigation waters. The addition of K significantly affected [Na], [K] and K:Na ratio in shoot. All other root parameters were significantly affected by the K addition. The Ca uptake by roots increased with saline irrigation. The P addition increased [P] in plant tissue. The Na uptake by root and shoot tissues was depressed with the addition of P and K fertilizers. The higher K uptake increased K:Na ratio in both shoot and root tissues. The values of EC, SAR and [P], [Na], [Ca], [Mg], [Cl] and ratios of Na:K, Ca:P, Cl:P and SO4:P in the soil leachates significantly increased with saline irrigation. The P addition had significant bearing on the soil pH, SAR, [P], [SO4], [CO3] and [HCO3] and Ca:P, Cl:P and SO4:P ratios in leachates. Saline irrigation significantly increased ECe, [Na], [K], [Ca], [Mg], [Cl], [CO3], SAR and ratios of Ca:P, Cl:P and SO4:Cl in saturated extracts of post-harvest soils. This study could suggest that the addition of P and K under saline-sodic conditions may affect salts and nutrients dynamics of the soil and was useful for crop productivity.

Potassium (K) and sodium (Na) coexist on the soil exchange complex and soil solution. Both cations may exert antagonistic or synergistic effects on mutual absorption and translocation within plants, particularly under saline and saline-sodic field conditions. This study investigated the role of K in alleviating the adverse effects of Na on wheat [Triticum aestivum (L), Iquilab-91] grown at two fields sites varying in salinity. The soils were moderately calcareous, weakly structured, mixed hyperthermic Typic Haplustepts. Site 1 was silty clay loam saline-sodic soil (ECe = 4.23-9.45 dS m-1) irrigated with groundwater with an ECiw value of 5.0 dSm-1 ; Site 2 was clay loam saline soil (ECe = 3.2-5.0 dS m-1) irrigated with groundwater with an ECiw of 2.4 dS m-1. Both sites were treated with 0, 50 and 100 kg K ha-1 applied as K2SO4 (41% K) fertilizer. Significant increases of 14 and 30% in grain yield were measured at both sites, and 35 and 54% increase in dry matter yields were observed in clay loam soil with the application of 50 and 100 kg K ha-1. Potassium application decreased leaf [Na] and significantly increased [K]; the K:Na ratio showed a positive correlation with yield. Soil analysis showed significant increases in [K], [Na] and SAR, while pH, ECe and [Ca+Mg] were not affected by the K fertilizer. The K2SO4 increased crop yield by mitigating the adverse effect of Na and would thus be an effective source of K for crop production in saline and saline-sodic soils.

Effect of composting on the extractability of plant nutrients in organic wastes is essential for their recycling in sustainable soil fertility management. Therefore, an experiment was carried out to determine the concentrations of total C, total N, extractable P, K, Na and B in five types of fresh and composted manures (namely buffalo, camel, cow, goat and poultry). Laboratory results showed that the extractability of elements varied considerably with the type of composted manure. Total C varied in the order cow > goat > buffalo > poultry > camel while total N was in the order buffalo > poultry > cow > camel > goat. Total C, total N, extractable K and Na decreased with composting, whereas extractable P and B increased. Goat manure had higher pH and EC values than cow and poultry manures whether in fresh or composted samples. A marked increase in the EC value was observed in composted manures, whereas pH was reduced with composting.